Field imagery alone rarely settles modern taxonomy. Confirmation required intensive laboratory work. Molecular biologists at the CUNY Graduate Center, working alongside international research partners, conducted multi-locus gene sequencing to determine whether the population represented phenotypic variation or a distinct speciation event.
The molecular teams sequenced both mitochondrial markers and nuclear loci from the collected tissue samples. The results were conclusive: the Likweli colobus diverged from its nearest relatives between 1.2 million and 1.8 million years ago. This split coincided with Pleistocene-era climatic shifts that altered the flow of the Congo River's major tributaries. As water levels changed, pockets of forest opened and closed, trapping ancestral primates within isolated habitats where natural selection shaped their unique physical and behavioral profile.